Composite

Part:BBa_M1523:Design

Designed by: Sarah Allred   Group: UtahState_BE5930_-_S11   (2012-03-23)


Protease with promoter, RBS, and two transcription stops


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 287
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 527
    Illegal NgoMIV site found at 761
    Illegal NgoMIV site found at 1013
    Illegal NgoMIV site found at 1217
    Illegal NgoMIV site found at 1261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 362
    Illegal BsaI site found at 1523
    Illegal BsaI.rc site found at 1076
    Illegal BsaI.rc site found at 1637


Design Notes

NA


Source

The coding sequence of this part is from a genomic sequence from Haloferax volcanii DS2, ATCC 29605. The promoter, RBS, and transcription stops are standard parts found in the parts registry.

References